Estudio Fitoquímico y evaluación de la actividad antioxidante In Vitro de hojas y flores de Passiflora ligularis.

Abstract

The photochemical study and evaluation of the antioxidant activity in vitro of leaves and flowers of Passiflora ligularis; in the Escuela Superior Politecnica de Chimborazo within the Research Group of Natural Products and Pharmacy (GIPRONAF) within the framework of the institutional project “A comparative study of the psychoactive activity of passifloras of the Chimborazo province”, were carried out with the collection and drying of the vegetable matter, the quality control of the leaves and flowers, a screening photochemical of extracts, a thin layer chromatography (TLC) in leaves and flowers were made. The quantification of flavonoids by high pressure liquid chromatography (HPLC) was conducted, it was determined the recruiter actvity of free radicals through the method of 1,1-diphenyl-2-picril-hidrazilo (DPPH). In the quantification of phenols and flavonoids the extract showed higher concentration was the ethanolic to 70% with 716.67 mg of gallic acid/g of dry leaves and 122.22 mg of gallic/g of dry flowers, while for flavonoids was obtained 54.33 mg of quercetin/g of dry leaves and 12.53 mg of quercetin/g of dry flowers, in HPLC results were expressed as a percetage of apigenin, quercetin and luteolin, obtaining for apigenin 0.0246% in leaves and 0.0538% in flowers, for quercetin 0.0730 % in leaves and 0,0575% in flowers and for luteolin 0.0026% in leaves and 0.0114% in flowers. The inhibitory capacity IC50 of the methanolic extract to 99.8% was 310.0 ug/mL in leaves and 4692.6 ug/mL in flowers. This study concluded that there is greater recruiter activity of free radicals in leaves tan flowers, and recommended to use different concentrations of DPPH for obtaining best results in the recruiter actvity of free radicals.