Actividad cicatrizante del extracto cerraja (Sonchus oleraceus l.) En ratones (Mus musculus).

Abstract

This research aims to evaluate the healing activity of sow thistle plant (Sonchus oleraceus L.) in mice (Mus musculus) of the Vivarium of Science Faculty in Escuela Superior Politécnica de Chimborazo, with the purpose of rescuing ancient knowledge and take medicinal properties that sow thistle provides the new alternatives for a possible development of phytomedicine which contributes to knowledge on society. In the qualitative analysis it was determined that this plant owns secondary metabolites in dry sample higher flavonoids intake, tannins, phenols and fresh lesser extent. It was performed a quality physicochemical analysis control of water alcohol extract of the sow thistle plant at different concentrations 20 %, 40 % and 80 % with a pH of 6.20 to 6.33, refractive index of 1.336- 1.347, main sample relative density 0.0662 g/ mL 20 % (1.0 g/ mL), 40 % (0.0865 g/mL), 80 % (0.0763 g/mL), total solids 1 %. The microbiological control in plates Petrifilm 3M (total coliforms, molds and yeasts), PCA “Plate Count Agar” (mesophilic aerobic) determining the absence of microorganisms. For the evaluation of healing 18 mice (Mus musculus) were used divided into 3 experimental groups inducing wounds 2 cm long, 2 mm deep and different treatments were applied considering the healing time the length of the scar, the results were analyzed by Anova and Tukey test with a confidence level of 95 % to determine the cell regeneration of the wond. It was applied a histopathological through a microscopic and macroscopic analysis. It is concluded that water alcohol extract of sow thistle plant ( Sonchus oleraceus L.) is effective and with greater effect in concentration with 80 % having a greater healing than the other treatments presenting a healing in 9 days. It is recommended to pharmaceutical companies to develop the production of phytomedicine of proven quality.