Evaluación de la actividad cicatrizante de geles elaborados a partir de extractos lipídicos, etanólicos y saponinas de dos variedades de quinua

Abstract

Evaluating the healing activity of gels made from lipid, and ethanolic Criollo Quinoa saponin and Tunkahuan White extracts was performed. This res earch was conducted in the B ioterio UNI VERSIDAD NACIONAL DE CHIMBORAZO, in the pharmaceutical industry and Laboratory of Phy tochemistry NEOFARMACO the ESCUELA SUPERIOR POLITECNICA DE CHIMBORAZO . In order to obtain a phytodrug directly involved in the healing process. Maceration techniques for the ethanol extract, soxhlet method for lipid extract and exhaustive extraction with n - butanol to extract saponins: methodologies for obtaining extracts were applied. Toxicity also Artemia salina and mice ( Mus musculus ) was evaluated. These were assessed by their behavior during 7 days. Quality control testing of raw material and excipient s is performed, evaluating physical parameters such as: pH, density, and microbiological extensibility. The gel was prepared at two concentrations 1% and 2%. The healing activity was evaluated in wounds in the back of the mouse (Mus musculus ), applying the gel twice a day and measuring the area of the wound for 15 days. The results were analyzed with ANOVA and DUNNETT'S lipid extract Quinoa Tunkahuan 1 % showed a decrease in the wound starting fr om 0, 878 cm 2 and arriving achieve an area of 0, 012 cm 2 . Whi le the lipid extract Tunkahuan 2 % saponin extract Quinoa Criolla Blanca 1% also have he aling activity areas reaching 0,0117 and 0, 0163 cm 2 . The lipid extract gel with 1 % Quinoa Tunkahuan had higher healing activity, the presence of fatty oils that prom ote the healing process. It is recommended that further studies for the pharmaceutical industry to market this type of herbal medicines.