Evaluación de la actividad hipoglucemiante del extracto hidroalcohólico de hojas y fruto de Synsepalum dulcificum en ratones (Mus musculus) con diabetes inducida por Estreptozotocina

Abstract

The objective of the research was to determine the hypoglycemic activity of the hydroalcoholic extract of leaves and fruit of Synsepalum dulcificum (miracle fruit) in Mus musculus mice with streptozotocin-induced diabetes (STZ). To obtain the extracts, the plant material was macerated in 70% ethanol for 72 hours. Followed by which he concentrated on rotary evaporation. In vivo analysis was performed by inducing diabetes with 150 mg / kg streptozotocin. Sixty-three mice were randomly distributed into nine groups of seven animals each, considering a first normoglycemic white group, the second positive group (acarbose), third negative control group streptozotocin and the fourth, fifth, sixth, seventh, eighth and ninth group was administered hydroalcoholic extracts of leaves and fruit of 30-60 mg / kg respectively, glucose levels were measured in hours and in days after the administration of the treatments; Later, the statistical analysis of the data is carried out in the MiniTab19 program, applying an ANOVA and the Tukey test to verify the existence of significant differences between the treatments administered. The in vitro tests that were carried out determined the presence of flavonoids, tannins and quinones in the phytochemical screening, which was corroborated with thin layer chromatography the presence of possible flavonoids. Giving as results so that the two doses used have the hypoglycemic activity xviii almost in its entirety in a time of 5 hours the induced hyperglycemia, being the dose of 30 and 60 mg / kg in leaves the most effective than the acarbose positive control. It can be concluded that the leaves could be used as a potential antidiabetic, and due to the presence of flavonoids it would present important antioxidant effects, which would subsequently prevent the triggering of the metabolic syndrome in the patient.