Validación de dosis con efecto ansiolítico del extracto vegetal de toronjil (Melissa officinalis) a través del empleo de un dispositivo electrónico automatizado.

Abstract

The objective of this research was to validate an automated electronic device for the determination of doses with anxiolytic effect of the lemon balm plant extract (Melissa officinalis) obtained through the collection, selection, drying and crushing of the vegetable matter, later the maceration was carried out by placing 50 g of plant in 500ml of ethanol to 70% during three days and it was concentrated until eliminating the alcohol. For the evaluation of the anxiolytic activity, thirty male mice were applied to which solutions of plant extract were administered at concentrations of 100ppm, 200ppm, 300ppm, Propylene Glycol 15% (Negative Control), Clonazepam (Positive Control). Neurological tests were applied for the evaluation of anxiolytic activity: light-dark room, elevated plus maze and open field. The data were captured in videos that were later processed by the programs initially used in ITOPI investigations for Light-dark room, Elevated plus maze; EMIS for Open Field and for the new software to validate called ANXIOLYTIC LAB. The data issued by ITOPI and EMIS were statistically analyzed in the SPSS program while the data expressed by the ANXIOLYTIC LAB software automatically ran the statistical tests. Finally, for the validation of the automated electronic device, the statistical results obtained both in SPSS and the ANXIOLYTIC LAB software were compared, concluding that the results issued by both teams are statistically similar. Regarding the anxiolytic effect, the doses of 100ppm, 200ppm, 300ppm showed significant anxiolytic activity in the three tests, with the 300ppm dose affecting the motor activity of the mice.