Determinación de la prevalencia de Brucella abortus por los métodos 2-Mercapto Etanol, Rosa de Bengala y Elisa en los trabajadores del Camal del Municipio de Riobamba.

Abstract

The main aim of the following investigation was to determine the prevalence of Brucella abortus among the slaughterhouse workers in Riobamba Municipality, through the Rose Bengal, 2-mercaptoethanol and ELISA methods. A total of 71 people had their blood drawn in an experimental trial conducted in a small percentage of an overall population. Socialization was put into effect in order to know important aspects of the Brucellosis disease. In addition, a survey was carried out to understand the risk factors to which this population is exposed and consequently, to be considered a vulnerable group. The tests were undertaken in the clinical laboratory at the – ESPOCH faculty of sciences and for this research was used the blood serum. In the Rose Bengal clinical outcomes was noted a zero degree of agglutination in the 100% of the population. Therefore, we have come to the conclusion that the test results have shown negative effects on the antigen-antibody reaction; all of this was taken into account to detect the presence of IgM antibodies and it was also performed the 2-mercaptoethanol method resulting in 99 % negative, 1 % positive and as a definitive test the ELISA method was applied, in which 96% was negative in IgG and 4% positive, in the case of IgM 99% negative and 1% positive. According to the survey results, 55% are aware of Brucellosis, 43% are not familiar with this disease and 2% refrains from giving an answer. All the staff are subjected to significant risk practices and most of them have been a great deal of time performing the same function, as to the protective clothing, they frequently wear the proper outfit to guarantee the bio-safety conditions. Most of the symptoms and signs of disease shown by the personnel is lower-risk. This indicates that the 62% of the working staff have gone through medical check-ups once a year. It is highly recommended to those people who work with animal fluids and internal tissues to always keep in mind the adequate bio-security measures as means of care and protection.