Evaluación in vivo de la actividad cicatrizante del extracto etanólico de Clinopodium tomentosum (Kunth) en lesiones inducidas en ratones (Mus musculus).

Abstract

In this experimental work, the healing activity of the ethanolic extract of the Leaves and flowers of Pumin (Clinopodium tomentousm Kunth) was verified in lesions included in mice (Mus musculus). The quality control of the dry plant matter and the ethanolic extract found within the reference values was carried out. The metabolites found by the phytochemicals were: steroids, oils, alkaloids, coumarins, resins, triterpenes, saponins, tannins, phenols, quinones, and flavonoids. The ethanolic extract was obtained by maceration of dried and ground leaves in alcohol at 96%; afterwards it was concentrated on a rotary evaporator up to total solvent dryness. We performed the quantification of phenols and total flavonoids in these trials were obtained 13.853 MgEQ/ml and 1.731 mgEQ/ml respectively which are relatively low. The evaluations of the mice, 25 male mice were used, which were subdivided into 5 groups: control of the ethanolic extract (25, 100 and 300 ppm) in a carbopol base gel. As a result of the healing evaluation the extract with greater healing effect was the 300 ppm of ethanolic extract of C. Tomentosum since this hill wound in about 10 days compared to the other treatments used as the positive control that it was closed the wound in 11 days. For this reason it is recommended in later studies to analyze other parts of the plant and the effect of the essential oil of Clinopodium tomentosum in order to determine if these can have a better healing and regenerated effect of the skin.