Comprobación de la actividad inhibitoria del extracto hidro-alcohólico de Piper peltatum sobre hialuronidasa.

Abstract

The objective of the research was to verify the inhibitory activity of the hydroalcoholic extract of Piper peltatum on hyaluronidase. The vegetal matter was collected and the crude drug and the hydroalcoholic extract quality parameters were analyzed. Through the photochemical screening, the presence of alkaloids, terpenes, phenols and flavonoids were determined. In the same way with the colorimetric methods, the phenolic and flavonoid compounds were quantified. Different contractions of the plant extract (1000; 250; 100; 50; 25; 12,5 y 6,25 µg/mL) were used for hyaluronidase inhibition test; a control group of maximum enzymatic activity and a negative target was used. It was determined that the best result in the inhibition of bovine hyaluronidase was at the concentration of 250 µg/mL with 81.13% inhibition; the most relevant results were obtained against snake venom cobra (Naja naja) at concentrations and more diluted 6,25 µg/mL with a 40,17% inhibition while in the Bothrops atrox species the activity was less significant at the concentration of 12,5 with 24,7% inhibition. The difference between the percentages of inhibition can be given by the genetic variability of the two species, and amount of hyaluronidase present in the venom. It is advisable to perform this investigation with venom from other snakes due to its traditional use in bites.