Evaluación de la actividad antiinflamatoria y citotóxica in vitro del extracto hidroalcohólico de hojas de Sigesbeckia serrata DC.

Abstract

The purpose of this research study was to evaluate the in vitro anti-inflammatory and cytotoxic activity of the hydroalcoholic extract of Sigesbeckia serrata DC leaves. For this, the quality parameters were determined through physical and chemical tests, both in the raw drug and the extract, a phytochemical screening was carried out through colorimetric and organoleptic tests, the content of total phenols expressed as gallic acid and flavonoids expressed as quercetin with spectrophotometric techniques was quantified. The antioxidant capacity was estimated through the DPPH radical uptake test, the anti-inflammatory and cytotoxic capacity was evaluated with the WST-1 assay for production of superoxides in activated neutrophils. It was determined that Sigesbeckia serrata presents in its composition alkaloids, triterpines, tannins, flavinoids, catechins, coumarins and resins. The content of total phenols was 74.48±0.41 mg / g of extract, while that of flavonoids was 60.43±1.24 mg / g of extract. This hydroalcoholic extract presented an IC25 of 524.70 ppm for its anti-inflammatory activity, 727.96 ppm for its cytotoxic activity, and 572.95 ppm for its antioxidant activity. It is concluded that it does not have an outstanding anti-inflammatory or cytotoxic potential compared to acetylsalicylic acid, nor an appreciable antioxidant capacity. It is recommended to carry out studies on the alkaloids that this species presents, and to complement this research with various in vitro and in vivo tests to determine its antioxidant and anti-inflammatory activity in other conditions.