Producción de un crudo enzimático (Celulasa, Xilanasa) por Aspergillus niger van Tieghem (ATCC® 6275™), utilizando residuos agroindustriales.

Abstract

In the current research was evaluated the production of an enzymatic crude (cellulase, xylanase) by Aspergillus niger van Tieghem (ATCC® 6275™) using three agro industrial residues; wheat bran, soy bran and brewing draff, through fermentation in a solid state (FES). The three-agro industrial residuess had a particle size of 0.05mm, the both first was submitted to an acid pre-treatment with H2SO4, while the brewing draff to pass by a malting process did not need of a chemical pre-treatment. Later a supplement was added a nutrients supplement in order to generate greater extra cells enzymes. Then it was inoculated a concentration of 6,49x109 spores.ml-1 of A. niger to each substrate, evaluating to the 72,120,168, 216 hours of fermentation, 3 repetitions were used, the substrates were incubated to a temperature of 27°C with a humidity of 80 % . The agro industrial residues more the nutrient supplement favoured the growth and the production of enzymes (cellulase, xylanase) by A. niger the best substrate was the brewing bran. The cellulase groups analysed were Endoglucanase with a time of 216 h incubation, was obtained a concentration of 2,843 UI.ml -1, for its determination was used the method of Carboxymethyl Cellulase, on the other hand, exuglucanase was also determined to obtain a maximum concentration of 6103 UI.ml-1, an incubation time of 120 h, this enzyme was analysed by the filter paper method and also the concentration of xylanase was obtained, being 7,570 mg/ml within 72 h of incubation.