Determinación de la actividad cicatrizante de la planta Salvia Real (Salvia sagittata) mediante lesiones inducidas en ratones (Mus musculus).

Abstract

The healing effect of the lyophilized of the Salvia real (Salvia sagittata) leaves extract in induced injuries of mice (Mus musculus) was determined. Quality control of the vegetal material and extract was carried out. Phenolic compounds, oils, alkaloids, triterpenes, steroids, resins and saponins were identified by phyto chemistry screening. Sprayed vegetal material was marinated in alcohol at 70%, then it was cocentrated in a rotary evaporator in controlled conditions a and eventually, it was lyophilized to get the lyophilized extract. Thin layer chromatography about flavonoids and essential oils was also carried out. Quantification of total phenols and flavonoids we got as results 843.889 ± 9.623 mg GAE/100g and 166.959 ± 1.045 mg QE/100g of lyophilized extract respectively. We used 36 mice with induced injuries, which were divided into six groups as follows: negative control (without treatment), two positive controls (antibiotic skin ointment and matico extract to 200 ppm) and Salvia sagittata extracts to concentrations of 25, 100 and 300 ppm. The application of these extracts was carried out by gel. It is concluded that gel of Salvia sagittata to 300 ppm gave a better healing effect because injuries were healed in nine days regarding 16 days of negative control and it was complemented with the hispatologic test. It is recommended to carry out an analysis of the root and stem of this plant to find out which one the most healing effect has.