Determinación de las actividades fotoprotectora, genotóxica y de irritación ocular in vitro del extracto hidroalcohólico de Lippia scorodonioides.

Abstract

The purpose of the current graduation work was to determine the in vitro photoprotective activity of Lippia scorodonioides hydroalcoholic extract at 20, 200 and 2000 ppm as well as the capacity of the extract to cause eye irritation and genotoxicity at different concentration, being 500 ppm the highest concentration. The conditioning and quality control of the raw drug was carried out. Through a phytochemical sieving, it was possible to identify the presence of phenolic compounds and flavonoids. The Lippia scorodonioides dry extract was obtained through a concentration process and the subsequent lyophilization of the hydroalcoholic extract. The total phenol and flavonoids, antioxidant capacity (using DPPH*), Sun Protection Factor (SPF) Mansur’s equation were quantified through spectrophotometric methods. The eye irritation capacity of the extract was determined by the HET-CAM and CAM-TBS method. The genotoxic activity was evaluated with the Vicia faba –seed micronuclei test. It was possible to determine the in vitro photoprotective activity through the UVB radiation-induced cell death on Escherichia coli ATCC 25922. The number of surviving UFC (Unit Forming Colonies) was detected in 30-minute intervals, during 3 hours. The total phenol and flavonoids content was 1.148mg GAE/100g dry extract and 0.30mg QE/100g of dry extract respectively. The antioxidant capacity was IC50 1004.89 ug/mL. The extract presented a sun protector Factor of 0.75 to 10 ppm unlike OMC and Pamidato that obtained values of 12.50 and 17.81 respectively. The hydroalcoholic extract presented a low photoprotective effect with a bacteria proliferation until 60 minutes less than the one presented by the OMC controls and Pamidato that was until 120 minutes. The Lippia scorodonioides hydralcoholic extract did not present nor eye irritation or capacity to induce genotoxicity. It is recommended to carry out more studies for Lippia scorodonioides since there is no any study on this species.