Microencapsulación de apigenina mediante coacervación compleja.

Abstract

The objective of this research was to prepare apigenin microcapsules by the complex coacervation method. The solubility of the active ingredient in three different oily vehicles (castor oil, almond and olive oil) was determined by spectrophotometry compared to a standard, the weight yield of the microcapsules obtained from 9 different treatments in which the proportions of polymer s, concentration of crosslinking agent (TG transglutaminase) and total reaction volume, the encapsulation efficiency of the microcapsules obtained and the hygroscopicity of the microcapsules of the valid batches. The oil vehicle in which the active ingredi ent has the best solubility corresponds to castor oil (14.146 ± 0.05 ppm), the treatment w ith the highest yield by weight of microcapsules was C5 (3.842 g) (apigenin dissolved in castor oil 4 mL, gelatin and gum arabic ratio 1: 1, TG concentration 15 U / g gelatin, total reaction volume 100 mL), the one with the highest encapsulation efficiency was C8 (82.52%) (apigenin dissolved in castor oil 4 mL, gelatin and gum arabic ratio 2: 1, TG concentration 15 U / g gelatin, total reaction volume 75 mL). Finally, the treatment that had a lower value of hygroscopicity was C4 (11.58%) (apigenin dissolved in castor oil 2 mL, gelatin and gum arabic ratio 1: 1, TG concentration 30 U / g of gelatin, total volume of reaction 100 mL). The best performance in terms of solub ility of apigenin was that associated with castor oil. The encapsulation efficiency has its best value when having proportions of gelatin and gum arabic 2: 1 and concentration values of TG 15 U / g of gelatin. However, hygroscopicity in these conditions has an intermediate behavior of approximately 16%. It is suggested to perform the apigenin release study from the obtained microcapsules.