Evaluación del efecto de microorganismos antagonistas en el control de la marchitez bacteriana (Ralstonia solanacearum E. F. Smith), presente en plantaciones de Eucalipto tropical (Eucalyptus urograndis) en la Hacienda Los Ángeles, cantón Buena Fe, provincia de Los Ríos

Abstract

The present investigation proposes: to evaluate the effect of antagonistic microorganisms in control of the Bacterial Wilt (Ralstonia solanacearum E.F. Smith) present in the plantation of tropical Eucalyptus (Eucalyptus urograndis) in Los Angeles farm, Buena Fe canton, province of Los Ríos. Company NOVOPAN DEL ECUADOR S.A. has the need of a research program on control of pests and diseases, pathogenicity tests conducted on plants of Eucalyptus urograndis under semi-controlled conditions using strains of R. solanacearum and the efficiency of commercial biotrop and biofung products also evaluated. The experimental used design was the Completely Random Design (DCA) with seven repetitions and an absolute control. The ImmunoStrip test used to verify that the plants were healthy, three doses (10, 30, and 50 ml) of bacterial broth inoculated and at 37 days all the plants showed the same symptoms of flaccidity, yellowing and wilting. Two methods of antagonism took place: Simultaneous inhibition and Dual culture with the commercial products face R. solanacearum where its control degree was zero, that is, its antagonistic capacity was none, the invasion of the surface of pathogenic strain and bio-controller potentially very bad both biofung and bioprot, based on what obtained in this investigation. The research paper recommends the use of specific isolates of Pseudomonas fluorescens of the PGPR type (Rhizobacteria) and with ectomycorrhizal fungi point to another form of biological control of the disease.

KEY WORDS: MICROORGANISMS ANTAGONISTS – PATHOGENICITY - CEPA - TROPICAL EUCALYPTUS.