Obtención de colageno de las patas de pollo con la aplicación de niveles de 2, 4, 6% de pepsina

Abstract

At the faculty of livestock Sciences from the Higher Polytechnic School of Chimborazo, collagen was obtained from the chicken legs with an application of 2%, 4% and 6% of Pepsin, with three repetitions each one, distributed under a completely random design. The results were considered by analysis of variance and the division of means according to Tukey. Stating that the use of pepsin affects statistically the physical-chemical and microbiological characteristic of collagen from the chicken legs. It is shown that when applying 6% of pepsin, the best responses were reported in total nitrogen (13%), higher percentage of protein (78%), density (0.37g/mL), conductivity (20.48mS/cm), and less amount of aerobic (42 UFC/g). As for the variable pH (5.28) of collagen, there are statistical differences, but this decreased as the pepsin levels increased. Therefore it is recommended to use 6% of the pepsin in obtaining collagen from the chicken legs, since the best physical-chemical and microbiological characteristic were presented.

Keywords: Extraction, Pepsin, Collagen, Conductivity.