Análisis de la expresión del gen metionina sulfóxido reductasa a (MSRA) en células foliculares bovinas en diferentes estadios

Abstract

At the autonomous University of Chihuahua-Mexico, evaluated expression of the Methionine Sulphoxide Reductase A (MSRA) gene was evaluated in samples of mature Cocs (Cumulus- Ovocyte Group) 1 and 2 and inmature 1 and 2 as well as mature Cumulus 1 and 2 and inmature 1 and 2 by complementary DNA synthesis and real-time polymerase chain reaction (qPCR). Data were analyzed using descriptive statistics, with three replicates plus a Negative DNA control; the results were subjected to a T-Student test at one (P ≤ 0.05). The data presented for the variables that expressed the gene did not show significant statistical differences;however, the presence of the MSRA gene was demonstrated in inmature Coc 2 and mature Coc 1 and 2. This is why the identification of the MSRA gene in bovine follicular cells could have a relevant impact in the oocyte maturation process, and that cells with the presence of the gene could be more efficient in discarding residues of cellular metabolic processes and raise the rate of blastocyst production in in vitro fertilization, as it is recommended to select Cocs that express the gene Methionine Sulfoxide Reductase A (MSRA) In vitro processes, due to its relevance in the elimination of oxygen species (EROS) in the process of bovine folliculogenesis.