Determinación de Buenas Prácticas de Producción de Ratones (Maus Musculus) en el Bioterio de la Escuela de Bioquímica y Farmacia

Abstract

The objective of the research is to determine the parameters of Good Production Practices of Mus musculus in the Biotherium of the School of Biochemistry and Pharmacy of the Escuela Superior Politécnica de Chimborazo. The macroenvironment and microenvironment samples were randomly selected, as well as the animals. The microenvironmental and macroenvironmental factors in which the experimental animals (Mus musculus) of the Biotherium of the School of Biochemistry and Pharmacy live during the period November 2009 - April 2010 were analyzed. The analysis of the factors. Macroenvironmental factors, temperature, relative humidity and sound have averages within the allowed range, however the light intensity at 10:00 am is 968.3 lux in room 2, this particular measurement is outside the established parameters. The results of the microbiological analysis in the environment, shelves, cages and feeders, have a concentration of more than 300 cfu/m3 , so it is necessary the urgent implementation of the guide of Good Production Practices of Mus musculus in the Biotherium of the School of Biochemistry and Pharmacy of the Escuela Superior Politécnica de Chimborazo, guide proposed in the present investigation. For mesophilic aerobes, the food has in all the parameters analyzed a high range of bacteria that is too numerous to count (MNPC) and the water sample contains 72 ufc/100mL for mesophilic aerobes, the range indicates up to 30 ufc/100mL, for total coli the result is 6.5 ufc/100mL the norms allow < 2 ufc/100mL. When analyzing the ectoparasites in the experimental animals, we found that 40% of the animals had few ectoparasites. Eighty percent of the animals have some type of parasites of which 30% have Strongyloides stercoralis larvae: some, 30% have Ameba histolyitica cysts: few, 10% have Giardia lamblia cysts: some and 10% have Giardia lamblia cysts: numerous. In animals in the analysis of fecal contents.