Determinación de la Sensibilidad in Vitro de Malassezia globosa Frente a los Hidrodestilados de Calendula officinalis, Rosmarinus Officinalis y Salix alba

Abstract

This research was carried out in the Clinical Microbiology laboratory of the Faculty of Sciences of the Escuela Superior Politécnica de Chimborazo. The objective is to identify the antifungal activity of three plants: Rosmarinus officinalis (A), Salix alba (B) and Calendula officinalis (C) and their mixtures (AB, BC, AC, ABC) for Malassezia globosa, causal agent of dandruff, since this disease is one that affects more than 50% of the world population, and we want to find a treatment of natural origin. We proceeded to isolate and identify the causal agent of dandruff in modified Dixon medium incubated at 32°C 1°C for a period of seven days, after which the strains were preserved in Dixon broth. To determine the antifungal activity, the Mitscher method was used, which consists of mixing the hydrodistillates with the Dixon medium and incubating the Malassezia globosa strains. The hydrodistillates of vegetables A, B and C are extracted by vapor entrainment. The results of "in vitro" determination of antifungal activity against the blank are positive for hydrodistillates of: A (100%), B(100%), ABC (33%, 33%, 33%), AB (50%, 50%), AC (50%,50%), as they have a similar reaction to the activity exerted by 2% ketoconazole on the strains. It is concluded that the above hydrodistillates have antifungal activity for the fungus Malassezia globosa.