Estudio Farmacognósico y Actividad Antimicrobiana (in vitro) de los Extractos Fluídos de Arrayán y Pumín y su Aplicacón en una Pasta Dentifrica

Abstract

The study of the components of Myrtle (Myrcianthes rhopaloides) and Pumín (Salvia squalens) and experimental evaluation of in vitro antimicrobial activity in native strains isolated from a saliva pool was carried out to provide an alternative for the treatment of periodontal infections, discarding the mandatory use of antibiotics. The fluid extracts of Arrayán and Pumín were prepared by percolation after quality control of the crude drugs, phytochemical screening and thin layer chromatography identified compounds with potential antimicrobial action, a screening by disk diffusion showed activity against Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Candida albicans; MICs and BMCs of the separate extracts and their combined effect were determined; a dentifrice with the best combination was formulated. The physical-chemical analysis of the drugs determined moisture and ash content within acceptable limits. Phytochemical screening established the presence of aromatic essences such as borneol, 1,8-cineole, eucalyptol, triterpenes, flavonoids such as quercetin, cynarin and rutin. The fluid extracts of Pumín and Arrayán analyzed independently showed a MIC of 1000 mg/mL and CMB of 500 mg/mL, the combined fluid extracts (1000:1000mg/mL) showed better activity; they were active against Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes with the exception of Candida albicans and would have potential applications for oral hygiene and dental therapeutics.