Determinación de la actividad antiinflamatoria de la planta Clinopodium tomentosum mediante inhibición de edema plantar inducido por caragenina en ratas Rattus norvegicus.

Abstract

It was studied anti-inflammatory activity of the ethanolic extract from Clinopodium tomentosum induced by plan edema in test animals. The extract was elaborated by maceration with alcohol to 96% with a crushed and dried sample. It was then perfomed the quality control of the raw drug and physical- chemical quality control of the extract. It was done the thin layer chromatography (TLC). By means of spectumphotometrical techniques, it was quantified the amount of phenolic compounds with Gallic acid standard, the total flavonoids with quercentin standard. The evaluation of the antioxidant capacity was done by means of using the method with radical DPPH (2,2-diphenyl-1-picryhydrazyl), Gallic acid was used as standard antioxidant, these results were valued as media inhibitory concentration (MIC). The anti-inflammatory activity was determined by means of induction of plant edema induced by carrageenan to 1% with the test animals (Rattus norvegicus), six groups were formed, each one with 4 rats which were given 25, 100 and 300mg/kg of extract, the inflamation area of the rat´s leg was measured from time zero until the seventh hour, the inflammation percentage was analyzed by using Microsoft Office PowerPaint; for the statiscal analysis, it was used the SPSS system with ANOVA test for one factor and TukeyB.

In the phenols quantification it was gotten 6,68% with 66,832 mgEQ/mL, while total flavonoids 0,92% at a concentration 9,240 mgEQ/mL, regarding the antioxidant capacity of the extract, its MIC is 47,751 µg/mL with a percentage of 64,25% of the free radicals catching capacity. The anti-inflammatory activity was determined, being the doses of 100 and 300mg/Kg of extract the ones that significantly reduce the inflammation of the rat´s leg. This study is related to Eupatorium gloutinosum which presented such activity. It is concluded that the extracts with higher concentration present a good anti-inflammatory activity, wich benefit future studies regarding its to toxicity.