Determinación de la actividad antiinflamatoria de Kalanchoe pinnata mediante inhibición de edema plantar inducido por carragenina en ratas (Rattus norvegicus).

Abstract

The aim of this investigation was to determine the anti-inflammatory activity of the leaves of Kalanchoe Pinnata (Lam.) by inhibition of plantar edema induced with carrageenan in rats. Successive macerations were carried out with 96% ethanol by rotary evaporator concentration. A soft extract was obtained, which was subsequently conveyed as suspension in doses of 25,100 and 300 mg / Kg. To evaluate the anti-inflammatory capacity of the alcoholic extract, an inflammation in the right paw of the rat was induced with the aid of carrageenan to 1% Intraperitoneal (IP), the measurement was performed with the Image J .It is a program which allows to calculate the area of the inflamed paw at different hours, the treatment of the data was done with the statistical program IBM SPSS statistics v.23. The extract obtained had a yield of 17.79% and a pH of 2.55; with phytochemical screening, the presence of alkaloids, triterpenes, steroids, phenols, tannins and flavonoids. It also exhibited a concentration of 1,586 mg EQ / mL, 57, 723 mgEQ / mL, 21,319 μg / mL in the quantification of flavonoids, phenols and antioxidant activity respectively. This study concludes that the extract of Kalanchoe pinnata (Lam.) has antiinflammatory activity, which is more visible at time 7 and effective at the dose of 300 mg / kg,

which was administered orally and has a percentage of inflammation of 60.05%. An ethno botanical and toxicological study of the naturalized species in Ecuador is recommended.