Determinación de la actividad antiinflamatoria de la planta Salvia quitensis mediante inhibición de edema plantar inducido por carragenina en ratas Rattus norvegicus.

Abstract

The anti-inflammatory activity from the ethanolic extract of Salvia quitensis was determined by inhibition of plantar edema induced with carrageenan in rats (Rattus norvegicus. The Salvia quitensis extract was obtained by maceration with 96% alcohol, and its filtration and concentration, too. With that the solvent used was removed entirely. Quality control on both the drug and the ethanolic extract was performed. The flavonoid content and total phenolic were carried out through spectrophotometric techniques. The evaluation of free radical capacity was based on the method proposed by Brand-Williams. The inflammation was induced by carrageenan injection of 1% under the plantar aponeurosis of the right back leg of the rat. Twenty four rats were used for developing this experiment. The same were chosen at random and divided into 6 groups. Diclofenac Sodium, was applied as positive control, Eupatorium glutinosum as reference extract and Salvia quitensis with 25 mg/kg, 100 mg/kg and 300 mg/kg. The treatments were administrated 30 minutes before induction of edema through via oral. The volume was measured in the leg of the rat with the ImageJ program. The percentage of inflammation was obtained with Microsoft Office Excel 2013. For the statistical analysis, the ANOVA test and TukeyB were applied. All treatments in some way had anti-inflammatory activity because the percentages obtained were below the value of the negative control. The soft ethanolic extract of S. quitensis at a concentration of 100 mg/kg showed a great antiinflammatory activity with 49.5%, inflammatory percentage. This value is very closer toward positive control and the reference extract regarding the other dosages 25 mg/kg and 300 mg/kg whose percentages were higher. It is recommended to develop a toxicological study from the ethanolic extract of S. quitensis for completing this the research study.