Determinación de la dosis efectiva para actividad ansiolítica del extracto etanólico de hojas de Passiflora edulis Y Passiflora quadrangularis en ratones Mus musculus mediante administración oral.

Abstract

The objective of this research was determined the effective dose of Ethanolic extracts of Passion Fruit (passiflora edulis) and badea (passiflora quadrangulis) in mice (Mus musculus) by oral administration; the study was realized in the Lab of Natural Products and Vivarium of the Escuela Superior de Chimborazo. The plant material was collected, dried, extracted with ethanol with 85% and then it was lyophilized. The flavonoids of the extracts were analyzed by thin layer chromatography (CCF) and quantify by Spectrometry UV. Anxiolytic activity was assessed in 60 mice, which were divided into groups of five mice. Five groups were given five different doses (25, 50, 100, 200, 300 mg/kg). One per white group (15% propylene glycol) and one control group (clonazepam 0.5 mg / kg). The anxiolytic activity was evaluated with four tests: elevated cross labyrinth (EPM), open field (oft), light / dark room and myorelaxant activity (chimney).The measured parameters were: number of entrances to arms, percentage of time in open arms, time in clear room, recognized pictures, time in the center, time that it takes to leave the chimney.The percentage of extraction of dry leaves of P. edulis was 9.96 % and P. quadrangularis 12, 32%. For (CCF) thin layer chromatography it was identified: isovitexin, vitexin -2 ", -0-glucose in extract of P. edulis and in P. quadrangularis isovitexin and/or isovitexin -2 ", -0- Rhamnosid and/or vitexin -2 ", -0- Rhamnosid. The lyophilized extract of P. edulis contains 81, 0 ± 0, 06 FTEQ/g of Flavonoids and P. quadrangularis of 81, 6 ± 4, 18 FTEQ/g. It is concluded that the dose with higher anxiolytic activity of the lyophilized extract of P. edulis was 25 mg/kg and 100 mg/kg P. quadrangularis; therefore effective doses are formed for the treatment of anxiety in mice. It is recommended to determine the acute toxicity from 300 mg / kg.