Determinación de la dosis efectiva para actividad ansiolítica del extracto etanólico de hojas de Passiflora manicata y Passiflora tripartita en ratones Mus musculus mediante administración oral.

Abstract

The increase of mental disorders related to anxiety were the objective of the investigation to determine the effective doses of the ethanol extracts of wild Taxo Passiflora manicata and common Taxo Passifflora tripartite in Mus musculus mice by oral administration. This was a study, which was carried out in the Laboratory of natural products and vivarium of Escuela Superior Politécnica de Chimborazo. The plant material was collected, dried, extracted with ethanol 85% and lyophilized. The flavonic part of each extract was analyzed through Thin Layer Chromatography (TLC) and quantified spectrophotometrically in the UV. The anxiolytic activity was evaluated in 60 mice grouped in five per group. It was administered five doses of extract (25, 50, 100, 200, 300 mg/kg) one per group. A white group (propylene glycol 15%) a control group (clonazepam 0.5 mg/kg). The anxiolytic activity was evaluated with Elevated plus maze (EPM), open field test (OFT), light/dark room and muscle retaxant activity (chimney). The measured parameters were the following: number of entrances to arms, percentage of time in open arms, time in clear room, number of squares traversed, time of staying in the center, time that it takes to leave the chimney, The percentage of dry leaves extraction of P. manicata was 4.22% and P. tripartite 5.01%. By Thin Layer Chromatography TLC were identified Schaftoside apigenin in extract of P. manicata and in P. tripartite the isovitexin and isoorientin. The lyophilized extract of P. manicata leaves contains 661,667 ±3,803 FTEQ/g flavonoids and the P. tripartite 157.529±.877 FTEQ/g. In conclusion, the dose with higher anxiolytic activity of the lyophilized extract of P. manicata was 300 mg/kg and 25 mg/kg for P. tripartite; constituting effective doses for the treatment of anxiety in mice and it is suggested a more specific pharmacological study with receptors.