Determinación de la actividad fotoprotectora in vitro de los extractos de Chusquea sp.

Abstract

In the present research work determined the protective activity of the plant species Carrizo (Chusquea sp), which has a photosynthetic mechanism C4 and development in several ecosystems. Over time has produced an increase of UV index in Ecuador, resulting in a higher incidence of skin cancer. To evaluate the protective quality carried in order the cell death method against UVB radiation by using Escherichia coli ATCC (29522) as well as the ethanolic extract of the plant used as a barrier, the Pamidato and Octylmethoxycinnamate (OMC) controls at concentrations of 20 ppm, 200 ppm and 2000 ppm; thus forming the experimental unit. On the other hand, it observed surviving bacterial colonies at different exposure times and transformed into percent cell wall for comparison. The sun protection factor (SPF) was determined through the Mansur methodology; at the same time the amount of total flavonoids in the ethanolic extract. The thing layer chromatography (TLC) was performed using a solvent system for the identification of flavonoids, besides it verified that a concentration of 2000 ppm of the ethanolic extract of Chusquea has a rate of feasibility of 10, 62 % presenting the mayor protective capacity and obtaining a SPF of 6, 11 to 10 ppm. In the chromatography, 3 possible compounds were identified such as: Quercetin-3-O-gentiobiside, kaempferol-3-Orhamnnoglucoside and Isorhamnetin-3-O-glucoside. It the determined that this plant species has a medium level of protection, which could be used in cosmetic pharmaceutical formulations however, it recommends to complement with other protective substances to increase its range of protection.