Análisis toxicológico agudo del extracto etanólico de Passiflora ligularis y Passiflora mixta sobre Rattus norvegicus por vía oral.

Abstract

The acute toxicolgical evaluation of ethanolic extract of Passiflora ligularis and Passiflora mixta on Rattus norvegicus (albine rats). It was held in the Laboratory of Natural Products and Bioterio from of the Escuela Superior Politécnica de Chimborazo, Riobamba, Ecuador. The biological material used was grown-up albino rats of the species Rattus norvegicus, 36 female rats in total; and leaves of Passiflora ligularis and Passiflora mixta. The leaves of Passiflora ligularis and Passiflora mixta were collected in Penipe, Chimborazo province, dried-out at 38°C, pulvericed and macerated in ethanol at 85%. The product of the maceration was sonicated, filtered out, concentrated and finally freeze-dried. Four experimental groups of 6 rats each were conformed; a control group and a target group were designed and, to which were given propylene glycol at 15% because it was the vehicle that was used. Later, blood samples were taken from the rats to show that the values were within the normal range. A dose of 300 mg/kg of the freeze-dried ethanolic extract was given to the first group and 2000 mg/kg to the fourth group, on a single occasion orally; its behavior was monitored for 14 days. Clinical observations were made daily and at the end of the investigation determinations of hematological parameters and clinical biochemistry were performed. To carry out the histopathological study, brain, heart, kidney, liver and lung were taken out. Toxic signs were evaluated before and after the test, hematological indicators were valued (Red blood cells, white blood cells and platelets) and clinical biochemistry (Alanine aminotransferase ALT, aspartate aminotransferase AST, bilirubin, creatinine, urea). No mortality was observed nor clinical or hematological alterations that show a possible toxic effect of the extracts. It is recommended to continue with the toxicological study at chronic and sub chronic level to rule out any kind of liver toxicity of the 300 mg/kg dose.