Determinación de la dosis efectiva para actividad ansiolítica del extracto etanólico de hojas de Passiflora ligularis y Passiflora mixta en ratones Mus musculus mediante administración oral.

Abstract

The objective of the research was to determine the effective dose of the ethanol extracts of Granadilla Passiflora ligularis and curuba Passiflora Mixed in Mus Musculus mice by oral administration; the study was carried out in the Natural Products and Bioterror Laboratory of the Politecnic School of Chimborazo. The plant material was collected, dried, extracted with 85% ethanol and subsequently lyophilized. The flavonoids from the extracts were analyzed by thin layer chromatography (TLC) with mobile phase of acetic acid; formic acid; water (100: 11: 11: 26) and quantified by UV spectrophotometry. Anxiolytic activity was evaluated in 60 mice, which were divided into groups of five mice, five groups were given five different doses (25, 50, 100, 200, 300 mg/kg) one by group, a blank group (propyleneglycol 15%) and a control group (clonazepam 0,5 mg/kg). The Anxiolytic activity was assessed with four tests; Labyrinth in elevated cross (EN), open field (OFT), light / dark room and myorelaxant activity (chimney). The parameters measured were: number of entrances to arms; percentage of time in open arms, time in clear room, charts covered, time in the center, time it takes to leave the chimney. The percentage of dry hours extraction of P.ligularis was 5.27% and P. mixed 4.96%. By TLC was identified as isovitexin, vitexin, vitexin-2 O glycosyl in extract P. mixed and the schaftoside compound in P. ligularis. The ffeeze-dried extract of P. ligularis leaves contains 307.203 + 4.237 FTEQ/g of flavonoids and that of P. mixed of 379.54 + 4.54 FTEQ/g. It was concluded that the dose with higher anxiolytic activity of the lyophilized extract of P. ligularis was 10 mg / kg and 200 mg / kg for P. mixed; And thus constitute effective doses for the treatment of anxiety in mice. It is recommended to determine the acute toxicity in rats from 300 mg/kg, with liver profiles, and pathophysiological studies of liver, kidney, lung.