Detección de anticuerpos contra Brucella spp en muestras de sangre de los estudiantes de la Escuela de Bioquimica y Farmacia de la ESPOCH.

Abstract

In the present investigation a protocol of diagnosis of Human Brucellosis is presented with the objective of detecting antibodies like Brucella spp in blood samples of the students of the School of Biochemistry and Pharmacy of the Higher Polytechnic School of Chimborazo. A previously prepared and validated survey was applied for the collection of personal and epidemiological data, prior to obtaining the blood sample, a letter of acceptance of informed consent was provided. A population of 140 students underwent a rapid screening test with Rose Bengal antigen (RB); a semi-quantitative serological test was performed on a clean glass plate in which 40 μl of serum and 40 μl of antigen were placed, homogenized with an applicator, then the plate was shaken for four minutes with rotational movements, the results obtained were confirmed with Micro-agglutination test 2- Mercapto-ethanol only to 16 students who fulfilled risk factors in the applied survey; observing the results macroscopically the screening detected 0% of positive patients, concluding that the students of the School of Biochemistry and Pharmacy are not infected with the pathogen, therefore the university students have consumed dairy products that are free of the bacteria, and it is recommended to carry out the same study in a cattle-raising area of Chimborazo where there is consumption of unpasteurized dairy products in order to obtain more specific data from the province.