Evaluación de la eficiencia de las bacterias lipolíticas de rumen de vaca para la degradación de aguas residuales con grasas y aceites.

Abstract

The objective of this research was to evaluate the efficiency of cow tureen lipolytic bacteria for the degradation of wastewater with and oils. It was developed in the city of Riobamba in the Biotechnology and Animal Microbiology Laboratory of The Escuela Superior Politécnica de Chimborazo. For the isolation and inoculation of these bacteria, the following methodology was applied; samples of the luminal liquid were collected for the isolation of the bacteria, aerobic, anaerobic and in different agars were sown in order to verify the best conditions for the development of these microorganisms. Then a colony count and gram stain were made. Once the best medium and the bacteria for working were chosen, the process of making a massification of the bacteria continued. For the inoculation of the bacteria, the pH of the water was measured pH 3.8 and this was very acidic for the growth of these microorganisms, since they grow at a pH of 6-7, so it was decided to add sodium bicarbonate to raise the pH range. It was worked with two control groups and two units to which the bacteria were inoculated. All units were incubated in aerobic and anaerobic environments at a temperature of 37 O C. Then 13 and 28 days were allowed to perform biochemical oxygen demand analysis of BOD5, dissolved oxygen, microbial growth, fats and oils. At the end of the process, the anaerobic medium was the best for the development of these bacteria as it was possible to reduce fat by 52%. In the aerobic medium, aerobic control group also reduced fats, but not to a great extent. In the anaerobic control group, the fat concentrations remained the same. It is recommended to carry out the study for more than 28 days to know how the bacteria continue their development.