Determinación del medio de cultivo adecuado para la magnificación de la producción de Saccharomyces cerevisiae (CE-1118) y liofilización del producto obtenido con fines comerciales.

Abstract

This research was targeted the determining the appropriate culture medium for the magnification of the production of Saccharomyces cerevisiae (CE1118 and freeze-drying of nutrients, discarding some not favor the growth of yeasts, however the formulation witch was used molasses (sugar source) and alfalfa (fluent of micro elements and vitamins) was the most efficient for obtain a yeast dough. The colony forming units (CFU) and corresponding mass was obtained by counting using the camera Nuebauer reached a value of 7.63 (log 10) CFU / ml, along the limiting source for the growth of these was the sugar was determined, being 18ºC Brix and 25ºC +/- 1ºC for 181 hours of incubation the optimal conditions to achieve a considerable mass of yeast. The freeze drying as a means of preserving yeast, favors the maintenance of the viability, the smell or characteristic taste of the same, as there is no damage from the effect of heat on the final product and when rehydrated keeps most of their original weight. From a mass of 70.54g, subjected to drying at 35ºC for 72 hours obtaining as a final product 39.88g of semi dry yeast and 29.81g of dry yeast, ie a yield of 17.17% it is considered good. It is recommended obtain the mass of Saccharomyces with the same formulation of the culture medium, but managing other factors that could influence the growth and reproduction as temperature, incidence of light, and other sources of nutrients with higher content of phosphorus and nitrogen.