Evaluación comparativa de la actividad ansiolítica de los extractos de hojas y flores de Passiflora ligularis y Passiflora mixta por administración oral en ratones Mus musculus.

Abstract

The aim of the research was to determine the in vivo anxiolytic activity of the extracts of leaves and flowers of P. ligularis and mixed P., administered orally, using laboratory mice Mus musculus strain BALB/c, and applying various pharmacological tests for this effect. It was prepared using extracts: ethyl acetate, methanol and water; in a ratio of 10g of dry panta by 50mL of solvent. The extracts were obtained by maceration for 24 hours; subsequently, they sonicated for one hour, and taken to dryness. For evaluating the anxiolytic activity, each extract was reconstructed with propylene glycol 15% v/v. There were prepared at a concentration which guarantees a dose of 50mg/kg. Finally, experimental measurements for each individual were recorder in four pharmacological tests: elevated plus maze (EPM), open field test (OFT), four light/dark and muscle relaxant activity. Clonazepam used with reference anxiolytic drug. To evaluate the toxicity of the extracts it was performed test on Artemia salina. The extract was the most toxic methanol extract of P. mixed flowers because median lethal concentration (LC50) was less than 10 ppm, while the extract was presented greater security ethyl acetate flowers P. ligularis, since its LC50 was of 797. The most significant results in terms of anxiolytic activity were presented by extracts: methanolic flowers and mixed P., P.ligularis in testing elevated plus maze, leaves methanolic of P. ligularis in the test four light and dark, watery P.ligularis flowers in the stack test. A study using different doses of extracts is recommended, to determine the minimum effective dose exhibiting a significant anxiolytic activity.