Evaluación de la capacidad antioxidante y antiinflamatoria de la combinación de tinturas a base de Matico (Eupatorium glutinosum) y Acíbar de Sábila (Aloe barbadensis).

Abstract

Antioxidant and anti-inflammatory capacity of the combination of tinctures based matico (Eupatorium glutinosum) and aloe vera acibar (Aloe barbadensis) is analyzed. Tinctures are produced by maceration with 40% alcohol aloe vera acibar and 38% matico, from these combinations were prepared in different proportions: 30:70, 50:50, 70:30 respectively. A control of physical, chemical and microbiological quality was performed; the cromografia of thin layer (CTL) was performed. With the use of spectrophotometric techniques and the phenol content it was quantified total flavonoids. Evaluation of the antioxidant capacity test was performed by the radical 2.2-diphenyl-1-picrylhydrazyl (DPPH *); It was used as standard antioxidant gallic acid. The results were expressed as mean inhibitory concentration (MIC). The anti-inflammatory activity was evaluated by inducing plantar edema carrageenan, 21 rats (Rattus norvegicus), divided into seven groups, 300 mg / kg of dyeing was administered to each animal, the amount of inflammation was measured from 0 to 6, the results were analyzed with the ANNOVA and Tukey statistical tool. Quantification of phenols is obtained aloe vera acibar tincture 30% matico 70%, has the highest number with 7,417mgGAE/mL; dyeing matico and the highest concentration of flavonoids with 0.457 mg equivalents of quercetin/mL. Tincture more antioxidant activity is the tincture of matico with 2085.46 g / ml which has the lowest CIM. As for the anti-inflammatory activity it is defined dyeing matico as presents a higher activity with 39.386% efficiency, less than thrown by the positive control value. It is concluded that mothers and combinations tinctures not have good antioxidant and anti-inflammatory activity because their concentrations are high to produce an effect. It is recommended to evaluate the ethanol extracts of dyes evaluated, to enhance its effect.