Evaluación ansiolítica comparativa por solventes de los extractos de hojas flores de Passiflora manicata y Passiflora tripartita mediante administración vía oral en ratones Mus musculus.

Abstract

Passiflora tripartite and manicata are little studied species in the country, being considered only as exotic plants, flavonoid contentare for study to evaluate the anxiolytic effect. The aim of the research was to evaluate anxiolytic three types of extracts from the leaves and flowers of Passiflora species both in mice (Musmusculus). The study was conducted in the vivarium of the Faculty of Sciences ESPOCH and focuses on assessing the anxiolytic effect in four models in vivo; elevated plus maze (EPM), open bonnet test (OFT), test light/ dark and field test fireplace, Clonazepam was used as a standard drug (O.OS mg/kg) plus cytotoxic activity was studied with Artemia salina technique. In the EPM model the leaf extract p. aqueous tripartite increased the number of entries into the open arms (p <O.OS). The leaf extract of P. tripartite in methanol induces a muscle relaxant effect demonstrated in the increase of time from the test fireplace and evidenced by a decrease in fear of light in the pattern of light/ dark field (p <O, OS). While the cytotoxicity assay showed high toxicity extract sheet P. manicata in ethyl acetate as its LCSO was less tan 100 ppm and safest was the methanolic extract of leaves of Passiflora tripartite with LC5O 675, 48 ppm. Concluding, methanolic extract of leaves of P. tripartite administered at a concentration of 50 mg / kg apparently has the best effect and receptor activity are attributed to the presence of three flavone compounds. the cytotoxic study in mice is recommended investigations with higher doses of the extract in mice to assess the anxiolytic + optimal effect, as well.