Aplicación de técnicas de biotecnología reproductiva en la sincronización de estro e inseminación artificial en ovinas mestizas

Abstract

In UAIOCC of Tunshi Experimental Station, College of Animal Science – ESPOCH, synchronization of estrus in crossbred sheep, artificial insemination and semen quality was evaluated. It features macro and microscopic study, being the Ram 1 (Poll Dorset) the better semen quality over the Ram 2 (Rambouillet). To evaluate two estrus synchronization protocols, using ½ implant new and reused, associated with estradiol benzoate (BE), Prostaglandin F2α (PF2α) and equine chorionic gonadotropin (eCG) in crossbred sheep first and second birth, with IA (fresh semen). They were distributed in 4 treatments at the rate of 5 animals per treatment: T1 and T2 females first covenant second parity females, ½ NOR “New” + 2 mg BE (Day 0) + 0,5 mg F2α + 2 mg eCG (Day 7) was applied, during 9 days, for T3and T4 with females with similar characteristics and the same protocols that were made in T1 and T2 except for ½ NOR implant that was reused; on days 10, 11 and 12, they estrus observations were made and proceeded to IA. No significant differences between treatment were observed for the interval between the removal of the implanted and the onset of the estrus (T1: 20,71h; T2: 20,46h; T3 y T4: 19,72h; ± 1,08h, P>0,05); taking greater efficiency in the occurrence and duration of estrus (T3: 34,80h y T1: 34,00h; ± 1,21h, P>0,05) and 100% to the presence of estrus and pregnancy; and cost per pregnant female and synchronized to Protocol 1 ($ 30,37) and Protocol 2 ($ 27,30). Demonstrating the efficiency of the use of new and recycled NOR implants, we recommend it´s use on farms of small and medium producers of sheep´s.