Evaluación de la actividad cicatrizante del extracto de hojas de arrayán (Myrcianthes hallii), in vitro por inhibición de la hialuronidasa e in vivo en heridas inducidas en ratones (Mus musculus).

Abstract

The investigation objective consisted of evaluating the in vitro cicatrizing activity through inhibition of hialuronidase in vivo in induced wounds in mice (Mus musculus) of the myrtle( Myrcianthes halli). A dried up and pressed simple of the plant was taxonomically identified in the Herbarium of the Escuela Superior Politécnica de Chimborazo. The collection and drying up of the vegetal matter was carried out; later, the quality of the leaves previously pulverized was performed; the phytochemical sieving of the extracts which was carried out with different solvents: dietlic ether, alcohol al 96% and distilled wáter was developed; the hydrometanolic extracts were obtained through an extraction process by soxhlet ehere the favonic fraction was obtained and the chromatography in thin layer of leaves was conducted. Different treatments were used for the flavonic fraction of leaves, which were (5, 10, 20, 40, 60 and 80 mg/mL) for the essays of hialuronidase inhibition; a negative blank and a máximum enzyme activity control were used; for in vivo 5 treatments were applied these being: Control (+)= treated with Lamoderm Cream, Control (-)= blanks,Groups X, Y and Z = treated with extracts at concentrations of 40, 60 and 80%. The results obtained in the bovine hialuronidase inhibition of the cobra venom, Naja naja, were satisfactory in the leaf treatments at dosages of 80mg/mL with a 54.70% and 65.72% inhibition of more tan a 50 % respectively while in vivo the cicatrization time and the final length of the scar are consedered. The results were analyzed statistically with a 95% confidence interval by means of the Anova and Tuckey tests. It was concluded that the higher the dosage of flavonic fraction of leaves of Myrcianthes halli the higher percentage of hialuronidase inhibition, similar to in vivo.It is concluded that extracts at 60 and 80% possess an effective cicatrizing activity in a 12-dy time due to the presence of flavonoids and hence it offers a better cicatrizing effect in wounds. It is recommended to continue with chronic toxicity analysis so that the vegetable could be commercialized as such or its active principles in a pharmaceutical way.