Estudio fitoquímico y evaluación de la actividad antioxidante In Vitro de hojas y flores de Passiflora quadrangularis.

Abstract

It was made a phytochemical and evaluation study of the antioxidant activity In Vitro of leaves and flowers of Passiflora quadrangularis. It is collected the species in the community La Victoria. The analyses were made in Escuela Superior Politecnica de Chimborazo, within the Group of Investigation of Natural Products and Pharmacy (GIPRONAF) in the frame of the institutional project “Comparative Study of the psychoactive of passion flowers of the Province of Chimborazo”. The plant was conditioned, the quality control of the drug was made and the phytochemical screening. The study was focused in the thin layer chromatography (TLC) to elucidate composes of flavonic type, the quantification of phenols and flavonoids by espectrophoyometry UV and flavonoids of high performance liquid chromatography (HPLC), the determination of the catching capacity of free radical by means of 1,1 difenil-2-picril-hydrazile (DPPH). The best results of the espectrophotometric quantification was the ethnolic extratc to 70 % in leaves with a percentage of 13.11 %, in flowers 7.25 % of total phenols expressed as gallic acid for flavonoids in leaves and flowers: 1.18 % and 0.54 % of total flavonoids were found, expressed as quercetine. In the hydrolized flavonic fraction was quantified through HPLC in leaves 0.0057 % of apigenine, 0.00105 % of luteoline and 0.0103 % of quercetine, and in flowers 0.00125 % of apigenine, 0.00015% of luteoline and 0.0012 % of quercetine. In the catching capacity of free radicals the máxium concentration of the inhibitory average IC50 was obtained, from the metanolic extract of leaves to 5993.49± 3.6 ug/mL to 50%. By means of the making of this study, it is concluded that the extract of leaves present a better antioxidant capacity. It is recommended modifying the concentrations of DPPH and of the antioxidant to determine how to act these radical to diverse concentrations.