Análisis Microbiológico de los quesos frescos comercializados en el mercado Simón Bolívar (San Alfonso) de la ciudad de Riobamba.

Abstract

The microbiological analysis of fresh cheeses sold in the central market "Simon Bolivar (San Alfonso)" from the city of Riobamba, Chimborazo province aims to determine the microbiological quality of this dairy product consumed by the population; The sampling strategy was to analyze fresh cheeses seven marketing positions, sampling was performed in triplicate for each sample for three consecutive Saturdays in the month of December 2015 corresponding 05.12.19, by reference to the Standard NTE INEN 0004: 1984. For quantification of the microbial load Total coliforms, Escherichia coli, Staphylococcus aureus and aerobic mesophilic bacteria was analyzed; by qualitative techniques by Petrifilm TM and Ecuadorian Technical Standard microbiological control of food for the determination of coliforms by the most probable number technique detection; It was handled by the method of successive dilutions to facilitate quantitation (10-1hasta10-3), and after incubation presences colony forming units CFU / mL confirmatory testing microorganisms were Mannitol agar, eosin methylene blue and Plate count agar. The results of the research were: aerobic mesophilic bacteria (6.29 x 103, 4.96 x 106, 1.43 x 106 CFU / mL); Staphylococcus aureus count (4.29 x 103, 1.73 x 105, 6.99 x 104 CFU / mL); number of total coliform (4.19 x 104, 1.19 x 105, 2.29 x 105 CFU / mL); number of Escherichia coli (1.13 x 104, 3 x 104, 1.46 x 105 CFU / mL) values ​​compared against reference values ​​showing the NTE INEN 1528-2012 standard are in values ​​greater than the limits microbiological established. It is concluded that the present microbial load in fresh cheeses exceed the limits permitted by the relevant standard, therefore these products are unfit for human consumption by the poor quality of them. It is recommended to run a job with the Ministry of Health and the relevant supervisory authorities and health surveillance, continuous monitoring of microbiological analysis with technical personnel, trained to consider this issue as a public health problem, indicated in the statistics showing the MSP, to take control measures to protect the health of consumers.