Determinación de la estabilidad y viabilidad de Trichoderma harzianum Rifai en cinco sustratos usados para la elaboración de un biofungicida en formulación líquida

Abstract

Stability and viability of a biofungicide prepared in liquid formulation with five substrates basis on a native Trichoderma harzianum strain was evaluated, the CCCh-p1 strain was identified based on their morphological, cultural and molecular characteristics. With the obtained result the strain of Trichoderma harzianum was chosen and the solid substrate was replied (rice husks + broken rice) which was used for the preparation of formulations with five substrates the same were stored at 4ºC, 18ºC and 22ºC. Subsequently, the stability of the product was determined through the spores quantification using a Neubauer chamber for 60 days. Viability was evaluated in time by micro-culture method and the economic analysis of the treatment was performed. The CCCh-p1 strain was selected for all these processes in this research. It was found that the product stability influences with the type of substrate used in formulation and temperature showing that the most effective treatment is the substrate of wheat flour stored at 22ºC with a spore concentration of de 3,22x10 9 CFU/mL (Colony forming unit per millimeter) in latency phase. Regarding to the feasibility of treatments about time, an efficiency of 88.47% in the formulation prepared with banana substrate for 60 days of storage was obtained. The economic analysis showed that there is no significant variation in the product price. That is why the price is affordable for the producer. The use of biological products are recommended because they contain native strains and are prepared from a substrate which have the protein content that requires the fungus for its development.