Comprobación de la Actividad Cicatrizante del Extracto de Hojas de Lengua de Vaca (Rumex crispus) en heridas inducidas en ratones (Mus musculus).

Abstract

The healing activity of the ox-tongue leaf extract (Rumex crispus) in wounds induced in mice (Mus musculus) was proved, for which the quality control of both the dry drug and the extract was performed, phytochemical screening determined the presence of flavonoids, tannins, anthraquinones. Thin layer chromatography for flavonoids was carried out. By UV spectrophotometry the presence of total flavonoids expressed as quercetina was quantified; a concentration of 5.4 mg/g of leaves hydroalcoholic extract of Rumex crispus was obtained; in quantification of total phenols the Folin- Ciocalteu method was used and a concentration of 7 mg/g are expressed as gallic acid (GAE) was obtained. To be able to check the healing activity 24 BALB/c mice divided into 6 groups of 4 each were used; drug protocol used was (-) control on which no treatment was applied, 2 controls (+) to which was applied neomycin sulfate + prednisolone acetate alcohol and 40% respectively, Group 1 leaf extract Rumex crispus 25%, Group 2 extract at 50% and finally Group 3 extract at 75%. It was observed that the extract that provided best results was the one of Group 3, in 9 days healing appeared as well as the histopathological analysis better quality of scar tissue was obtained. It is recommended this research to be done with mixtures of species to thereby observe whether enhance the effect.