Evaluación Microbiológica de las aguas termales del balneario las Peñas, cantón Baños, provincia Tungurahua.

Abstract

A microbiological evaluation of the hot Springs Spa Las Peñas, Baños town, Tungurahua Province, in order to determine the microbiological quality of the source and ensure the health of those who come to this resort. It began with the measurement of physical-chemical parameters of the affluent, using multiparameter Hannah. The microbiological analysis was performed with two samples of the water hole and swimming pool, under the standard INEN 1105 Waters. Sampling for microbiological examination. Microbial identification was performed on Petrifilm Aerobic plate, E. coli / Coliform, Staphylococcus, mold and yeast, using 1ml of sample each. After flooding the colonies, they were selected the most representative to make the necessary chimes to obtain pure clones which underwent Gram staining and biochemical test: oxidase, catalase, mobility and rust fermentation. A bacteria that could not be identified underwent test Kligler, citrate, urea and SIM (Sulfide Indole Mobility). And finally the strains that despite all the studies were still not identified were submitted to Mycrogen system. The analysis resulted in a water hole contaminated with Gram negative bacterium corresponding 33% to Cardiobacterium spp and similar Methylobacterium mesophylicus percentage. Meanwhile the pool also contaminated whit Gram negative strains reported Aeromonas veronny bio veronny, this being the most common bacterial species isolation. Thereby establishing that exist in these waters a predominance of Gram negative pathogenic bacteria of sanitary interest, considered as indicators of human and environmental contamination. The decentralized Autonomous Municipal Government of Baños town is recommended to perform microbiological analysis Bathrooms newspapers to control the quality parameters of the thermal spa waters penalties and protect the health of those who come to the resort.