Evaluación de la actividad diurética del extracto de chuquiragua (Chuquiraga jussieui) en ratas (rattus norvegicus).

Abstract

The present research was the evaluation of the diuretic activity in Chuquiragua (Chuquiraga jussieui) extract en rats (Rattus norvegicus), conducted in the laboratory of Phytochemistry, laboratory of instruments and Bioterio of the Faculty of Science of Polytechnic School of Chimborazo. A previous study of the plant was conducted as: a quality control of Chuquiragua and extrac, phytochemical screening, chromatographic analysis on silica gel plates and quantification of total flavonoids by the method of AlCl3 using standard routine. For evaluation of the diuretic activity, they used 15 rats weighing 200 g to 300 g which were divided into 5 experimental groups, three for each treatment; chuquiragua extract at doses of 100 mg/Kg, 200 mg/Kg, 400 mg/Kg, to white rats administered saline, and for a positive control it used furosemide (20 mg/Kg), the total volumen of urine and electrolytes were measured. Finally toxicological analysis was performed by measuring different parameters. Phytochemical screening confirmed the presence of flavonoids, saponins, micilages, triterpenes and/or steroids, tannins, alkaloids, reducing sugars, phenols, bitter principles and fatty compounds. The diuretic effect of Chuquiragua (Chuquiraga jussieui) extract in three doses, with respect to white found in three treatments had very high diuretic effect, treatment three (400 mg/Kg) had a 638% being the most active. With regard to the positive control (furosemide) the treatments had lower activity, with 43% for the three treatment that had the highest percentage. The toxicological test evidenced no side effects of C. jussieui extract, so it can be regarded as nontoxic. It recommended that most pharmacological studies of C. jussieui of the therapeutic properties attributed to it and are not checked.