Evaluación del efecto cicatrizante del extracto de las hojas Bauhinia tarapotensis Benth (Pata de vaca) mediante lesiones inducidas en ratones (Mus musculus).

Abstract

To evaluate the healing effect of Bauhinia tarapotensis Benth (Cow Leg) by induced in mice (Mus musculus) injuries, this study was conducted in the laboratories of Natural Products and Bioterio from Science Faculty at the Polytechnic School of Chimborazo. Quality control of plant material were carried out which have the established standards. Extracts of 75%, 50% and 25% were prepared by the method of maceration with 96% alcohol, the phytochemical profile was determined in order to identify metabolites in the species, flavonoids were isolated by chromatography thin layer, in which quantified the total concentration of flavonoids and phenolic compounds by spectrophotometry. To ensure the quality of the extract were determined physicochemical parameters, organoleptic and microbiological characteristics. The healing activity was assessed by induced injuries on the backs of 24 mice (Mus musculus), they were divided into 6 groups: (untreated) Control -; (prednisolone acetate- neomycin sulfato) control + and (40% alcohol) Control +; extract treatment with 75%; 50% and 25%, which were administered topically. By statistical analysis were evaluated results with a confidence interval of 95%, it was found that extracts having healing activity, reducing the healing process. It concluded that 75% of alcoholic extract is effective compared to other research groups as their day of healing was 8 days, being this the most effective treatment. It suggests to Students from biochemistry and pharmacy carry out studies about Bauhinia tarapotensis Benth (Cow Leg), due to it has other pharmacological properties as a diuretic, digestive and antioxidants.