Evaluación de la actividad cicatrizante Iin Vitro del geranio (Pelargonium x domesticum L.H. Bailey) mediante inhibición de Hialuronidasa.

Abstract

The objective of this research was to evaluate the wound healing activity in vitro of geranium (Pelargonium x domesticum L.H. Bailey) through hyaluronidase inhibition. A dry and pressed sample was taxonomically identified in the Ecuadorian National Herbarium. After the sample was collected, the plant material was dried and pulverized. After that, the quality of leaves and flowers were supervised and subject to the phytochemical screening of the hydro-ethanol extracts. The hydro-methanol extracts were obtained through an extraction process called Soxhlet. The quantification of total phenols and flavonoids was done with the spectrophotometry UV. The flavonic fraction was obtained, and the thin layer chromatography (TLC) of leaves and flowers was also performed. Different treatments for the flavonic fraction of leaves and flowers were used, and were as follows (0.625; 1.5; 2.5; 5; 10 and 20 mg/mL) for the essay of hyaluronidase inhibition. A negative white and some control of the maximum enzyme activity. The results obtained in bovine hyaluronidase inhibition were satisfactory for the treatments with leaves and flowers at 0.625 mg/mL dose with a 90.89% and 92.49% of inhibition respectively. The results reached for the cobra poison Naja naja hyaluronidase inhibition were representative in more diluted doses 0.000000009 mg/mL of leaves and flowers with more than 50% of inhibition. It was concluded that with lower doses of flavonic fraction of leaves and flowers of Pelargonium x domesticum L.H. Bailey a higher percentage of hyaluronidase inhibition was obtained; therefore, it offers a better wound healing effect. It is recommend to do a further study about the flavonic constituents present in leaves and flowers of Pelargonium x domesticum L.H. Bailey so that the agent that causes the wound healing can be determined.