Evaluación De la actividad cicatrizante de geles elaborados a partir de extractos lipídicos y etanólicos de Sangorache (Amaranthus hybridus L) sobre heridas producidas en ratones (Mus musculus).

Abstract

It proposed to develop a gel from Sangorache (Amaranthus hybridus L) provided by the Autonomous National Agricultural Research Institute (INIAP), with the aim of helping in the healing process of wounds in mice (Mus musculus). Ethanolic extracts and lipid were obtained from grain and leaves of sangorache by applying the maceration technique, using ethanol and hexane respectively. The Soxhlet method was used for the lipid extract. Subsequently the phytochemical analysis of extracts was performed using the methodology described in OLGA LOCK DE UGAZ. The toxicity level was increased by determining DL 50 evaluating mice´s behavior for 7 days. After that the quality control of gels was performed following the guidelines of Pharmacopeia (USP 28 AND 35) OF THE United States. The healing activity was determined through wounds induced in mice and formulated gels in 1 and 2% according to each extract applying twice daily for a period of 15 days. The results were analyzed with ANOVA and DUNNETT´S TESTS SHOWING THE FOLLOWING RESULTS. The gel with lipid extract from Sangorache Grain in 2% presented a difference between statistical averages of 0.177 according to the control of trolamina. The Sangorache gel helped to improve the healing process that is why it could be more effective than trolamina, thanks to fatty oils which facilitate to the healing process. It concluded that the lipid gel 2% of Sangorache grain contributes to the healing process providing better response times. It is recommended that the pharmaceutical industry continues with the next phases of the research for this phytodrug gets commercialized.