Estudio Fitoquímico y Evaluación de la Actividad Citotóxica y Ansiolítica In Vivo del Canayuyo (Sonchus Oleraceus).

Abstract

Canayuyo (Sonchus oleraceus) is a specie Little studied in the country, Little appreciates for being considered “weed”, its content of flavonoids of the type apigenina and luteolina (Flavonas) as well as quercetina (Flavonol) is motive of study for the evaluation of the anxiolytic effect. The objective of the research was to evaluate the effect cytotoxic and anxiolytic of the hydroalcoholic extract of aerial parts of Canayuyo in mice (Mus musculus). The study focuses on the quantification of flavonoids totals by a method spectrophotometric and liquid chromatography high efficiency (HPLC), evaluation of cytotoxicity by isolated neutrophils method and evaluation of the anxiolytic effect and action gabaergic in mice (Mus musculus) in two models in vivo, elevated pluz maze (EPM) and open field test (OFT). Clonazepam was used as a standard drug (0.5 mg/Kg) and Flumazenil (1 mg/Kg) as inverse agonist for the evaluation of the action of the extracts hydroethanolics on GABA receptors. Through HPLC was determinated 2.6792% of total flavonoids, data contrasted with a 2.5224% obtained by spectrophotometry UV expressed as % of apigenina. A concentration of 200 ppm of extract showed 0% of cell viability. In the EPM model extracts increased the number of entries in the open arms (p < 0.05) and the time spent in the same (p < 0.05). Extracts induce an effect antimotactic, evidenced by an increase of locomotion in the central part of the OFT (p < 0.05). The activity on the receptors are not sufficiently clear, which is attributed to the presence of three flavones compounds without defined activity. It is recommended to perform subsequent investigations with cuts to level of the brain of mice in microtome to evaluate the level of different receptors by electron microscopy.