Evaluación y validación de un amonio cuaternario de quinta generación y un compuesto yodado en la desinfección de las áreas de producción de queso y yogurt en la empresa Prasol Lácteos Santillán.

Abstract

This research had as main objective to evaluate and validate a Quaternary Ammonium disinfectant of fifth generation "Pentaquat" and an iodined compound "Chemlok I-1.75", disinfectant that will be employed in the sanitization processes inside the areas of production of cheese and yoghurt as a possible alternative in case of a chemical resistance takes place, which have been normally used in the Dairy product Factory Santillan "Prasol". The phenolic coefficient (PC) is an official technique for disinfectants that compares the bactericide efficacy of the same in relation to the phenol, control strains such as: Escherichia coli, Staphylococcus aureus and Listeria monocytogenes, which were used by making dilutions of the disinfectants and inoculating in three times: 5,10 and 15 minutes. The bigger concentration was selected, which eliminates in 10 but not in 5 minutes, the microorganism about the same results of phenol; concluding that for its PC> 1 accomplishes effectively with its germicide activity. The diffusion technique in laboratory dish permitted to know the bactericide power of the analyzed disinfectant to distinct concentrations representing through the aura inhibitors, formed around de filter paper disc impregnated with the disinfectant concentrations in an agar that owns an active suspension of pathogen microorganisms (0.5 McFarland). By means of an analysis of the obtained results, it was considered to "Pentaquat" a concentration of 175 ppm and for "Chemlok I-1.75" 62 ppm as ideal concentrations able to eliminate the studied strains of bacteria, showing a high efficacy. Finally, the verification in situ was applied by using microbiological sampling of the cleaned surfaces for three consecutive days per each cleaning product, to the found suitable concentration, was analyzed the three types of microorganisms mentioned before with dehydrated colorimetric laboratory dishes and specific lists for the sowing as they were Petrifilm and Compact Dry in which no growth was observed, in consequence in considered the 100% of elimination, concluding on this way to the validated concentrations of the disinfectants accomplish with their germicide action being able to be used with no problems within the installations of the factory.