Comprobación de la actividad cicatrizante del extracto de hierba Mosquera (Croton elegans kunth ) en ratones (mus musculus).

Abstract

The curren is base don the healing activity of the Mosquera Herb (Croton elegans kunth), in the Bioterium of Sciences Collage of Escuela Superior Politécnica de Chimborazo , routed to know the goodness that give the plants from Ecuador few know in order to give a good applicability in the medicinal area, establishing a patter for the elaboration of phyto- medicaments in next researche. Through the phytochemical screening the presence of secondary metabolites was identified like the flavonoids, phenols and tannins that area capable to heal. Through physic and chemical tests of the hydroalcoholic extracts to different concentrtions to 20%, 40%, 80% of Mosquera Herb (Croton elegans kunth) its quality was determined in pH from 6,97 – 7,13, refractive index 1,0159 – 1,0580, relative desity 0,99 – 1,00, total sollids 3,39 – 3,89. Microbiological control presents absence in total coliforms moulds and yeasts. The verification of healing activity was used in 18 mice divided in groups of three, three mice (mus musculus) for positive control, three mice (mus musculus) for negative control and three mice (mus musculus) for the research per se, which were subjected to hurts in back of 2 centimeres length, then the different treatments were applied, to identify the treatment effectiveness through heling time and the reduction of hurt , the results with ANOVA and Tukey tests were tabulated, with trust level of 0,5%, that means that at least two of the treatments have healing difference respect to days. The results were based on the histopatholocical analysis that reveals the regeneration percentage of epithelial cells. It is concluded that the extract acted of better way was the hydro-alcoholic extract to 80%. It is recommended that next researches give a pharmaceutical way in order to industrialize a new medicinal product.