Estudio microbiológico de las aguas termales Reina del Rosario de Cununyacu, parroquia Pilahuin, provincia Tungurahua.

Abstract

Reina del Rosario de Cununyacu hot springs do not have a microbiological analysis, for this reason it was necessary to carry out a native and non-native microbiota study through the sowing in 3MTM Petrifilm plates as well as tests to identify microbes. The native microbiota was analyzed taking wáter samples resulting in the growing of 1 CFU/ml belonging to Bacillus, in order to determine the existence of non-native microorganisms causing the contamination, some wáter samples taken from the pool were analyzed, as a result some relevant microorganisms for sanitation such as: Escherichia coli 12 CFU/ml, Proteus vulgaris 1 CFU/ml, Providencia rettgery 2 CFU/ml, and colonies of Staphylococcus 325 CFU/ml were found. Apart from analyzing the presence of bacteria, it was also determined that the waterhole does not show the presence of molds and yeasts, while in one of the pools there was growth of these two types of microorganisms. Within the microbiological analysis the (+) and (-) Gram bacteria percentage living in these hot springs was also determined, this analysis resulted in a higher percentage of (-) Gram bacteria with 66.67% and a lower percentage of (+) Gram bacteria with 33. 33%. To complement the study some in situ measurements for pH, temperatura, conductivity, and total solids were carried out; the measurements of these parameters were carried out in the waterhole, except for the temperatura that was taken from the pool were the analysis was done. The results were: pH is 7.8, 40°C, 4519 µs /cm of conductivity and 2892.16 ppm of total solids for the waterhole and 36.4°C for pool; with this data it is concluded that it is necessary to adopt microbiologic and sanitary control measurements providing its user welfare and avoiding health pathologies.