Estudio microbiológico de las aguas termo-minerales del Balneario “Santa Ana” de Baños de Agua Santa-Tungurahua.

Abstract

In the present investigation the microbiological analysis of samples of hot springs, taken from the shed and a pool of Ecological Resort “Santa Ana”, located in the canton Baños de Agua Santa in the province of Tungurahua was performed. The study was conducted at the Laboratory of Biochemical and Bacteriological Analyzes of the School of Biochemistry and Pharmacy, Faculty of Sciences. They were isolated in total 17 bacterial strains shed and pool, of which most were Gram negative bacteria. 6 bacterial strains shed all Gram negative bacilli and 11 bacterial strains of the pool, of which 8 were Gram negative bacilli, 2 Gram positive bacilli and one cocci Gram positive were isolated. Of bacterial isolates it was able to identify to genus and 14 species bacteria through biochemical tests and a bacterial strains by the MicrogenTM GnA+B-ID System method. In the case of Gram negative bacteris isolated from the side, species Flavobacterium aquatile, Aeromonas eucrenophila, Aeromonas media, Shewanella putrefaciens were identified, the species found most frequently were average Aeromonas media and Flavobacterium aquatil. The species found in the sample pool were Aeromonas media, Aeromonas caviae and Aeromonas schubertii in the case of Gram positive bacteria genus Bacillus spp. They were found Staphylococcus aureus species, the species most frequently isolated was also found Aeromonas media. Count of molds and yeasts was performed by the method of 3M Petrifilm Plates, where a greater number of molds and yeasts were found in the sample pool, rare in the sample being shed. From the results it can be deduced that the thermal waters of the Spa “Santa Ana” are recommended for recreational use and its study is available to the Municipal DSG (Decentralized Self-Government) city of Baños de Agua Santa