Estudio microbiológico de las aguas termomedicinales del parque Acuático los Elenes, cantón Guano, provincia Chimborazo.

Abstract

The Microbiological Study of the Thermo Medicinal Water in the “Los Elenes” Aquatic Park, in Guano, Chimborazo Province was performed in order to determine the microbiological quality of the spring water and ensure the health resort visitors. The study began whit the water source physical and chemical factors control, using the Hannah multiparameter. The microbiological analysis was made with two samples, the water source and swimming pool, with the INEN 1105 Standar. Water. Sampling for microbiological examination. The microbial identification was conducted on Aerobic Petrifilm plates, E. coli/ coliform, Staphylococcus, mold and yeast, using 1mL of sample each. After the colonies expanded, the most representative were chosen to make the necessary chimes to obtain pure clones which were put through Gram staining and biochemical tests: oxidase, catalase, motility and Oxide fermentation, in addition, the Gram positive bacteria were inoculated into starch and gelatin. For those bacteria which could not be identified, the Kligler, citrate, urea and SIM (Sulfide Indole Movility) tests were applied. Finally, the strains which despite all the studies were still not identified were submitted to Mycrogen system. The analysis led to a free bacteria water source, in the pool there was 33% Gram poisitive gender Bacillus spp bacteria and 67% Gram negative gender Enterobacteriaceae bacteria, with Escherichia coli and Cedecea species 5, and gender Vibrio with Vibrio algilolyticus. Thereby determining that in this water there is a predominance of Gram negative pathogenic bacteria of sanitary interest considered as indicators of potential human and environmental contamination. It is recommended to the GAD Guano to hire professionals in charge of making permanent microbiological analysis to control the water quality parameters in Los Elenes, in order to protect the swimmers, who visit the resort, health.